Wood-degrading fungi such as T. versicolor possess complex detoxification systems that allow them to cope with specialized metabolites produced by plants. Although the number of genes encoding GSTs is particularly high in these fungal organisms, little information is available on their target molecules. By combining biochemical, enzymatic and structural approaches, interactions between polyphenols and GSTs of T. versicolor have been demonstrated. To carry out this study, we set up original experimental protocols to obtain the crystallographic structures of complexes between GSTs and polyphenols (iii). In particular, affinity crystallography coupled with spectroscopic data (figure) allowed the identification of a flavonoid from cherry tree as a ligand of T. versicolor GSTs involved in the mineralization of this species (iv). In addition, a systematic review showed agreement between solution interaction data and crystallographic structures of the TvGSTO3S isoform in complex with a variety of hydroxybenzophenones. To quantify this agreement, crystallographic methods developed in the “Modelling” theme of the team, in particular the Charger algorithm, allowed the evaluation of the electrostatic interaction energies of each of the ligands, which are found to be well correlated with the affinities obtained in solution (v). Of course, this approach relies on obtaining crystallographic structures of protein-ligand complexes. Sometimes it remains a difficult step, for instance in the case of complexes between plant GSTs and antocyanins, which form an important class of relatively unstable, water-soluble pigments (vi). Several studies report a role for plant GSTs in their transport into the vacuole. More recently, we have been able to demonstrate an interaction of plant GSTs with several other less water-soluble but more stable classes of flavonoids. A decade ago, GST interactions with flavonoids were suggested; we have now succeeded in characterizing them from a structural point of view. Interestingly, recent studies by our collaborators in Dijon on human GSTs corroborate our results. Thus, plant flavonoids which belong to the human diet and are considered beneficial to health, are probably linked to these GSTs whose exact role remains to be clarified.